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Not only the oxidative burst is responsible for oxidative stress

Female patients must have two negative pregnancy tests before starting treatment and are advised to use two forms of birth control or practice abstinence during the six-month period due to the risk of birth defects (this is the purpose of the iPledge program - to prevent unintended pregnancies while taking Accutane)

This experiment was repeated on separate days with male and female mice from different litters, with each group consisting of 68 mice, to ensure reproducibility ( n = 14 per group)