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Cell culture reagents, including Dulbeccos Modified Eagle Medium (DMEM) and fetal bovine serum (FBS), were obtained from Euroclone (Italy)

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Moreover, these data provide a metabolic avenue for inducing HR deficiency and promoting sensitivity to DNA-damaging agents

Cells were washed with PBS and fixed with 4% paraformaldehyde (PFA) for 10 minutes at 4C, permeabilised for 15 seconds in a liquid nitrogen bath, blocked in 1% BSA / PBS for 1 hour at room temperature, incubated with D4H*-mCherry (1:25 in 1% BSA / PBS) for 2 hours, washed with PBS and post-fixed in 4% PFA for 10 minutes at room temperature