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How Oral KPV Targets the Colon When you take KPV orally, it passes through the stomach intact and becomes active once it reaches the colon right where inflammation occurs

Cells were loaded with MitoTracker Red dye (Excitation/Emission: 579/599 nm) at 100 nM final concentration (37 C for 15 min) mounted in Live Cell Imaging Solution (Thermo Fisher), and viewed under a laser confocal microscope (LSM 510

The authors show that the drastic growth arrest of the sir1 mutant is partially rescued when glutathione deficiency is introduced via the cad2 mutation, which decreases glutathione levels to 30% to 40% of wild-type levels (Cobbett et al., 1998), or pharmacologically through treatment with the GCL inhibitor buthionine sulfoximine