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Consistent with this finding, ZZ1 is especially potent in the Ewing sarcoma TC-71 cell line, which has elevated YPEL5 expression 43 (Extended Data Fig

Funding This work was supported by the National Science and Technology Major Project (2022ZD0117700), the National Natural Science Foundation of China (32270759) and the Research Foundation for Postdoctoral (AUGA5710026124)

Alkylation was performed with 55 mM chloroacetamide (Sigma-Aldrich) for an hour at 37 C with vortexing