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Nonetheless, 3DVA of CTR complexes bound to either Cagri or CagriBB revealed transitions between the two major alternative conformations (Supplementary Fig
and group 8: mice administered Dihexa, both through injection to the gastrocnemius muscle
Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution
The calculator above will recompute the unit count for any vial size and reconstitution volume
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