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The mean percentage of BrdU-labeled cells for three cultures per treatment was used as an indicator of activation (entry into the cell cycle) and the subsequent proliferation activity of plated satellite cells, according to Tatsumi et al

The concern isn't copper per se but rather the uncertainty about how supplemental copper peptides might affect a developing fetus or nursing infant

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The concentration of L-glutamate was then calculated using a standard calibration curve, normalized to protein sample concentrations, and levels expressed as percentage relative to controls