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For transient transfection, plasmids were transfected into HEK293T cells using polyethylenimine (PEI, branched, Sigma-Aldrich, Germany)

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Ceramides:Restore the skin's natural barrier, locking in moisture and protecting against environmental stressors

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You should also avoid drinking alcohol for at least 24 hours

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In all cases, the clinical differential diagnoses included MM and/or melanocytic nevus

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