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Control of specificity of GRK2 PF2 antibody in immunohistochemistry and islets detection in WT and GRK2+/- pancreatic sections

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Extracellular PK was removed by washing, PK activity was quenched by addition of a chaotrope and heating to 98 C, and samples were snap-frozen

If nausea limits fluid intake early in treatment, smaller frequent sips and electrolyte-enhanced beverages (like low-sugar sports drinks or broths) can help maintain sodium and potassium while meeting fluid goals