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Materials and methods Strains and culture conditions Yeasts were maintained and grown either in YPD broth [1% yeast extract (Oxoid), 2% bacteriological peptone (Oxoid), 2% D-glucose], or in YNB broth [0.69% yeast nitrogen base without amino acids (Formedium), 2% D-glucose] supplemented as required with amino acids, uracil, 5-FOA (Melford), D-histidine (Sigma) or reduced GSH (Sigma)

(2019) Could we be overlooking a potential choline crisis in the united kingdom? BMJ Nutrition, Prevention & Health, [online] Available at: Di Buono, M., Wykes, L.J., Ball, R

Most people prefer taking glutathione and vitamin C in the morning, often on an empty stomach, to support absorption and provide antioxidant protection throughout the day