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Description

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Multiplex immunofluorescence staining was performed by the manufacturers protocol (Akoya Biosciences, Japan) with the following primary antibodies: -SMA (1:50, Abcam Cat# ab7817), p-mTOR (1:50, Abcam Cat# ab109268) and FoxO1(1:50, Cell Signaling Technology Cat# 2880)

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Limitations As with any study, ours has limitations

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Months 4-6: Sustained Vitality - Continued improvement with consistent use

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