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To produce the retrovirus, the HOXC8-overexpressing or the mock control vector was co-transfected with the packaging plasmids into the HEK293FT packaging cell line using Lipofectamine 2000 (Invitrogen, Carlsbad, CA)

Thirty-five-week-old MC4R mice were randomly divided into four groups with no blinding at one day before drug treatment, vehicle (saline, s.c.), semaglutide (0.5 mg/kg, s.c.), tirzepatide (0.05 mg/kg, s.c.), and retatrutide (0.05 mg/kg, s.c.) based on amounts of food intake from day 25 before drug treatment, body weight at day 2 before treatment, and fat mass at one day before treatment

Assessment of RNA quantity and quality was performed with an Epoch Microplate Spectrophotometer (Biotek)

Together, acetaminophen and alcohol can irritate the stomach and, in severe cases, cause ulcers, internal bleeding, and liver damage