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The membrane was washed then probed with the appropriate species-matched secondary horseradish peroxidase conjugated antibody (0.4 mg/mL diluted 1:2500 mouse anti-rabbit IgG (sc-2357, Santa Cruz Biotechnology, Dallas, TX) or 0.4 mg/mL diluted 1:2000 goat anti-mouse IgG (sc-2031)) diluted in 5% skim milk powder in TBST for 1 h at RT on a rocker

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Among initiators of GLP1 receptor agonists versus SGLT2 inhibitors, the direction of effect modification by age in two prior studies [16, 25] was conflicting, and the direction of effect modification by cardiovascular disease and sex in our study disputes prior evidence [16, 25]