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The reaction was started by adding 3 U of GR and was monitored by measuring the change in absorbance at 412 nm for 1 min

However, because these observations rely solely on immunohistochemistry, they indicate potential involvement rather than providing definitive mechanistic evidence, and molecular validation is required to confirm these pathways

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The body weight of adult mice was estimated at 25 g, and the intraperitoneal injection volume was 250 l