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Physiol Behav (1999) 67(5):67983

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Biological analyses were performed on fasting blood samples, measuring folates, B12, zinc, cooper, magnesium, glutathione redox status, and folate receptor-alpha autoantibodies using immunoassays and biochemical assays

Time course experiments were performed using a single ligand concentration to determine the peak response time point (3min) for the subsequent concentration response curves as previously described