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The disulfide bond between Pt GSTF8 residue C13 and GSH was reduced on-column with 20 column volumes of rPPS buffer (20 mM potassium phosphate, 200 mM NaCl, 10 mM DTT, pH 7.4), at a flow rate of 5 ml min 1 and the column was then washed with 40 column volumes of PPS buffer at a flow rate of 5 ml min 1 to remove free GSH
RNA extraction and Real-Time PCR analysis of cytokine expression After separation of DNA and RNA, the latter was further purified using the RNeasy Mini kit (Qiagen) following the suppliers instructions
The other important function of selenium is as an antioxidant
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