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Annexin V apoptosis and cell-cycle assays Cell apoptosis was measured 48 h after treating with A939572 or infected with lentivirus using the APC-Annexin V Apoptosis Detection Kit (BD Bioscience, NJ, USA) according to the manufacturers instruction

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Immediate medical attention is required if patients experience severe abdominal pain, particularly if localized, persistent, or accompanied by fever, which may indicate complications such as bowel obstruction, fecal impaction, or rarely, intestinal ischemia

Eat before drinking alcohol