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Neurons were then washed fast 3 times with a warm Tyrodes buffer (124 mM NaCl, 5 mM KCl, 30 mM glucose, 25 mM HEPES, 2 mM CaCl2, 1 mM MgCl2, pH 7.4)

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Neuroimaging shows perimenopausal women have reduced brain glucose metabolism in Alzheimer's-vulnerable regions the hormonal transition is a window for brain protection, not just symptom relief