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Discrimination index The preference for and therefore recognition of the novel object, the novel arm in the Y maze, or the mouse over the empty cage were assessed by calculating the discrimination index (Ennaceur & Delacour, 1988): The discrimination indices of all genotype and treatment groups were compared individually with a theoretical value of 0, which would indicate no preference for the novel or familiar object, Y maze arm, or cage with mouse over empty cage using a one-sample t test

Plasma insulin was measured using the High Range Mouse Insulin ELISA (Alpco), and plasma total cholesterol was measured using the Wako Cholesterol E colorimetric assay (Fujifilm) following the manufacturers instructions

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It was so scary, the whole situation