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succinogenes was over-expressed in the various mutants and MG1655 by transforming them with the plasmid pTrc99a- as carrying gshF as (Table 1)

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To measure the effect of the paramagnetic label, 1 H, 15 N and 1 H, 13 C HSQC spectra with long inter-scan delays (3 s) of 100 M 13 C/ 15 N-labeled p53 TAD2 in presence of one stoichiometric equivalent paramagnetically labeled FOXO4 FH were recorded before and after addition of 5 mM ascorbic acid