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Given a 95% confidence level (Z = 1.96) and a 5% margin of error (E = 0.05), and assuming a proportion (P) of 0.5 to maximize the required sample size, we calculated the necessary sample size using RaSoft sample size calculator (Raosoft, Inc., Seattle, WA, USA) [24] to be approximately 384
Interestingly, serum levels of IL-6 were similar in five-week-old animals of both strains, with elevated levels in 10-week-old SHR, which correlates with that found in children with ADHD [34]
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Amitriptyline can have also anticholinergic, arrhythmogenic, and behavioral side effects, thus it can cause constipation, dry mouth, sedation, QT prolongation, increased appetite
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