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in short, cells were stimulated in the manufacturers stimulation buffer for the indicated time period at 37 C, before addition of the chemiluminescent substrate and luminescence read after a further 60 min incubation at room temperature

For researchers concerned about cardiovascular risk, practical strategies to minimize TMAO production include supporting a healthy gut microbiome through prebiotic and probiotic use, monitoring TMAO levels if testing is available, and keeping total levocarnitine exposure within studied safety ranges

This means you eat less, without depriving yourself and without counting calories

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