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A 10 g cell pellet was resuspended in 30 ml buffer B (25 mM Tris-HCl, pH 7.5, 10% glycerol, 0.01% Igepal CA-630, 2 mM 2-Mercaptoethanol) containing 500 mM KCl and a cocktail of protease inhibitors (PIs) (5 g/ml each of aprotinin, chymostatin, leupeptin, and pepstatin A) and lysed by sonication (QSonica Q700) at an amplitude of 40 for two min with 20 s pulses

6 In other studies, certain avocado extracts known as unsaponifiables have been shown to improve osteoarthritis pain and overall disability in people with hip or knee osteoarthritis, and may provide preventive effects when taken in the earliest stages of osteoarthritis