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For quantitative analyses, ratio of cells positive for nuclear -catenin staining to total number of cells was counted as pixel intensity ratio positive for nuclear localization using Image J (NIH, Bethesda, MD, USA)

We showed that norepinephrine (NE, 50 M) or dopamine (DA, 50 M) exerted potent protective effect against glutamate-induced cytotoxicity, but this effect was not observed when other neurotransmitters such as histamine, -aminobutyric acid, serotonin, glycine and acetylcholine were tested

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