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The cells were treated with Hanks Balanced Salt Solution (Hyclone) or 1 mM DTT for 10 min before incubation in Hanks Balanced Salt Solution with different concentrations of GLP-1 (02 M) at room temperature for 20 min

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The differencesdelivery method, dosage and use to treat obesity are all minor modifications of earlier disclosures that were made by Novo Nordisk in the main compound patents for semaglutide as discussed above

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