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Description

J Biol Chem 287(13):98739886

You will need: Peptide vial: This contains the powdered peptide you will be reconstituting

While most approaches treat chemical sensitivity and MTHFR variants as separate problems requiring different solutions, the cellular health perspective recognizes they're interconnected challenges

Then cells were harvested by centrifugation (6,000 rpm, 15 min), resuspended in lysis buffer [Tris-HCl (100 mM, pH 8.0), NaCl (300 mM), imidazole (20 mM), TCEP (5 mM), and PMSF (1 mM)], and incubated on ice with 1 mg/mL lysozyme for 30 min