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Here we report the cryo-electron microscopy (cryo-EM) structures of CCK1RG s signaling complexes liganded either by sulfated cholecystokinin octapeptide (CCK-8) or a CCK1R-selective small-molecule SR146131, and CCK2RG q complexes stabilized by either sulfated CCK-8 or a CCK2R-selective ligand gastrin-17

We also conducted a parallel experiment involving these cells and a subset treated with 0.5 U of neuraminidase to remove sialic acid residues from the glycoproteins on their membrane surfaces 21

The neurons of the cortical cerebral, cerebellar region, hippocampus, and hypothalamus were counted in 10 different high-powered fields (HPF, 400x) and 3 to 5 serial sections of each sample were used to do the count as described in The field size was 0.24 m 2